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af3805 anti asc  (R&D Systems)


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    R&D Systems af3805 anti asc
    Af3805 Anti Asc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af3805+anti+asc/us12570735-645-42-45?v=R%26D+Systems
    Average 92 stars, based on 10 article reviews
    af3805 anti asc - by Bioz Stars, 2026-07
    92/100 stars

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    LPS induced cell pyroptosis, apoptosis and pro-inflammatory cytokines secretion in both IUA mice and cellular models. The expression status of <t>(a)</t> <t>NLRP3,</t> <t>ASC</t> and N-Gasdermin D, and (b) cleaved caspase-3 and Bax were detected by performing Western blot analysis. The pro-inflammatory cytokines generation and secretion were examined by (c) Real-Time qPCR in EPCs cells and (d-g) its supernatants were examined by Real-Time qPCR and ELISA assay. (h) Western blot was used to determine cell pytoptosis in EPCs cells. (i) MTT assay was used to determine cell viability in EPCs cells. (j) Generation and (k, l) secretion of IL-1β and IL-18 were respectively examined by Real-Time qPCR and ELISA. Individual experiment repeated 3 times, and * P < 0.05
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    LPS induced cell pyroptosis, apoptosis and pro-inflammatory cytokines secretion in both IUA mice and cellular models. The expression status of (a) NLRP3, ASC and N-Gasdermin D, and (b) cleaved caspase-3 and Bax were detected by performing Western blot analysis. The pro-inflammatory cytokines generation and secretion were examined by (c) Real-Time qPCR in EPCs cells and (d-g) its supernatants were examined by Real-Time qPCR and ELISA assay. (h) Western blot was used to determine cell pytoptosis in EPCs cells. (i) MTT assay was used to determine cell viability in EPCs cells. (j) Generation and (k, l) secretion of IL-1β and IL-18 were respectively examined by Real-Time qPCR and ELISA. Individual experiment repeated 3 times, and * P < 0.05

    Journal: Bioengineered

    Article Title: Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells

    doi: 10.1080/21655979.2021.2001185

    Figure Lengend Snippet: LPS induced cell pyroptosis, apoptosis and pro-inflammatory cytokines secretion in both IUA mice and cellular models. The expression status of (a) NLRP3, ASC and N-Gasdermin D, and (b) cleaved caspase-3 and Bax were detected by performing Western blot analysis. The pro-inflammatory cytokines generation and secretion were examined by (c) Real-Time qPCR in EPCs cells and (d-g) its supernatants were examined by Real-Time qPCR and ELISA assay. (h) Western blot was used to determine cell pytoptosis in EPCs cells. (i) MTT assay was used to determine cell viability in EPCs cells. (j) Generation and (k, l) secretion of IL-1β and IL-18 were respectively examined by Real-Time qPCR and ELISA. Individual experiment repeated 3 times, and * P < 0.05

    Article Snippet: Next, the PVDF membranes were incubated with the primary antibodies against NLRP3 (1:1500, Catalog MAB7578, R&D system, USA), ASC (1:1000, Catalog AF3805, R&D system, USA), N-Gasdermin D (1:1000, Catalog ab209845, Abcam, UK), GAPDH (1:2000, Catalog 2275-PC-100, R&D system, USA), cleaved Caspase-3 (1:1500, Catalog AF835, R&D system, USA), Bax (1:2000, AF820, R&D system, USA), Cyclin D1 (1:1500, Catalog MAB4314, R&D system, USA) and CDK2 (1:2000, Catalog AF4654, R&D system, USA) at 4 °C overnight.

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, MTT Assay